How would you measure telomere length?
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Telomere sciencell blog sciencell Genetics & Genomics assay kits

Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.

 

ScienCell exclusively offers qPCR-based assay kits for the accurate, simple, and fast telomere length quantification of various species samples:

 

Absolute

Includes a reference gDNA sample with known telomere length. This kit can be used to calculate a sample's actual telomere length in kb.

For more information, please visit

 

Relative

NO reference gDNA sample included. Can be used to compare the telomere length of samples in fold change.

For more information, please visit

 

PRODUCT CHARACTERISTICS:

  • Unique: specially modified primers only available at ScienCell
  • Accurate: highly specific and qPCR efficiency close to 100%
  • Reliable: NO detectable primer dimer formation after 28-30 cycles
  • Informative: telomere length expressed in kilobases (Cat. #8918 only)
  • Sensitive: as low as 0.1 ng of gDNA required
  • Convenient: NO special treatment of gDNA necessary
  • Rapid: results can be acquired within 1 hour
  • Robust: suitable for a high-throughput setup

Figure 1. qPCR amplification curves using serially diluted telomere repeats as template.

Figure 2. Derivation of qPCR efficiency of Telomere primer set.