Molecular Biology
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- December 20, 2024
What is DNA/RNA Isolation?
DNA/RNA isolation, also referred as nucleic acid purification, is the process of extracting and purifying DNA or RNA from biological samples like cells, tissues, and body fluids.
The purpose of DNA/RNA isolation is to obtain pure nucleic acid samples free from contaminants and inhibitors. These contaminants can include proteins, lipids, carbohydrates, and other cellular debris that can interfere with downstream applications.
Isolation kits, such as those offered by ScienCell, provide standardized and efficient methods for DNA/RNA isolation. These kits typically use spin column-based technologies that utilize specific buffers and reagents to lyse cells, bind nucleic acids to a membrane, wash away contaminants, and finally elute purified DNA or RNA.
Here are just a few examples of how purified DNA/RNA can be used in research and clinical settings:
- Polymerase Chain Reaction (PCR): Amplifying specific DNA sequences for various applications, including gene cloning,
