Technical Tips for Successful Primary Cell Culture
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- May 31, 2018
When working with primary cells, it is important to remember that they are not cell lines and should be treated with care. At ScienCell, we specialize in primary cell culture and we are very familiar with the common problems researchers encounter when culturing them. We have compiled a list with 13 of the most common problems that researchers encounter when culturing primary cells.
Mistake #1: Being unfamiliar with the primary cell types being cultured.
Correction #1: It is very important to know the morphology of primary cells and to be aware of the morphology of potential contaminating cells.
Mistake #2: Primary cells are 100% pure.
Correction #2: Primary cells are rarely 100% pure so it is essential to pay close attention to cell morphology and to not allow cells to overgrow.
Mistake #3: Thawing a vial of primary cells in a water bath for longer than necessary.
Correction
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