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Many primary cells have difficulty adhering to uncoated glass and plastic surfaces, especially in low serum or serum-free conditions. For primary cells, it is necessary to use a substrate coating to enhance cell attachment. There are many different substrates to choose from and each type can have dramatically

Recently, bone marrow mononuclear cells (BMMC) have been in the spotlight for having the potential to treat a variety of diseases including neurological disorders. Why are BMMC so exciting and do they really show treatment potential? BMMC have regenerative capabilities and are a heterogeneous population

Despite being essential for any experiment involving DNA replication, people rarely give primers a second thought.  To coincide with the launch of our new qPCR gene reference tool, we’ll be giving them a little more of the praise they deserve.

In this next installment on how to improve your qPCR gene profiling (see 6 Tips to Improve qPCR for previous discussion), we would like to discuss the limitations of melting curve analysis and PCR inhibitors.

Tip #3. Melting curve analysis is NOT sufficient for assessing qPCR product specificity.

In

Choosing which media to use with your primary cells can be daunting. Whether you are replicating a formula from a publication, optimizing your own supplemented classical or nutrient media, or purchasing commercially available specialty media, it is important to understand how this choice will affect

American Heart Month Sponsor: The American Heart Association (http://www.heart.org/HEARTORG/)

Heart disease is the leading cause of death for men and women in the United States. Every year, 1 in 4 deaths are caused by heart disease.

The good news? Heart disease can often be prevented when people

Gene expression analysis is critical for understanding the transcriptome profiles of primary cells and how they directly influence the cells’ functionality. In addition, the transcriptome profile can affect cell proliferation, differentiation, senescence, or apoptosis. Traditional reporter-gene assays

qPCR array provides a quick, powerful and sensitive approach for gene expression profiling. To help our customers acquire accurate and consistent results, we are happy to share some tips for better qPCR.

Tip #1. Good primer design is the key to a successful qPCR array.

The success of a qPCR array depends

Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical role in scientific advancements, yet researchers have become increasingly cautious when interpreting

When working with primary cells, it is important to remember that they are not cell lines and cannot be treated the same. Because ScienCell has extensively working with primary cells, we are very familiar with the common problems researchers encounter when culturing them. Here are six common mistakes