Tag Archives: sciencell

ScienCell's Human Astrocytes (HA) - Immunostaining for GFAP, 200x.

Not All Primary Cells are Created Equal

Primary cells, which are isolated directly from tissue, show normal cell morphology and maintain many of the important markers and functions seen in vivo. Primary cells, though, have a finite lifespan and limited expansion capacity, so it is critical to use primary cells at low passage for your research.

Day 9 post-embedding: Endothelial cells stained with VWF (green) and pericytes stained with NG2 (red), 200x

Welcome to the 3rd Dimension

Cell culturing is a widely used technique to grow cells outside of their natural environment using artificial environments and controlled conditions that has become indispensable to scientific research.  The applications… Read more »

pseudogene timeline

Hidden Treasure in “Junk DNA”

3 Reasons Why You Should Take “Pseudogenes” Seriously in Your Research Protein-coding DNA, or “functional” genes, only accounts for up to 2% in the human genome. Until recently, the remaining… Read more »

Perspectives on Cancer

Perspectives on Cancer

Cancer is a collection of over 200 diseases where the only common denominator is rogue cells1,2.  The ways in which a cell can go rogue is so varied that cancer… Read more »

Comparison of Cell Viability, Proliferation and Apoptosis Assays

To obtain reliable data, assessing the health of cultured cells prior to any assays is essential. Furthermore, many cell-based assays require quantification of cell growth. Cell health and growth can be determined by quantifying cell viability, proliferation, or apoptosis

Rat Neurons-hippocampal #R1540

Neurological Disease Therapy through Regulation of Reactive Oxygen Species Levels

ScienCell’s Rat Hippocampal Neurons Used in Recent Neurological Disease Therapy Research Published in Scientific Reports Journal Neurons are dynamically polarized cells responsible for electrochemically transmitting information throughout the nervous system. Scientists have characterized hundreds of neuron… Read more »

Mouse Microglia #m1900

Novel Molecular Insights into Classical and Alternative Activation States of Microglia

ScienCell’s Mouse Microglia Used in Recent Microglial Activation Research Published in Molecular & Cellular Proteomics Journal Microglia are a type of macrophage-like glial cell in the central nervous system (CNS) and they function as the brain’s… Read more »

6 tips to improve qPCR

6 Tips to improve qPCR (Part 3 of 3)

In this final installment on how to improve your qPCR gene expression profiling (see 6 Tips to Improve qPCR: part 1 and part 2 for previous discussion), we will discuss… Read more »

Choosing the Right Substrate

Many primary cells have difficulty adhering to uncoated glass and plastic surfaces, especially in low serum or serum-free conditions. For primary cells, it is necessary to use a substrate coating… Read more »