Monthly Archives: November 2020
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- 十一月 12, 2020
Isolation of high-quality nucleic acids from biological material is a key factor in successful molecular biology research. While approaches vary, most methods aim to do the following: (1) disrupt the cellular structure, (2) denature and inactivate other macromolecules (e.g., proteins, enzymes, etc.), and (3) separate nucleic acids from cell debris and any contaminants. Some common approaches include organic extraction (e.g., phenol-chloroform-isoamyl alcohol method), differential precipitation (e.g., “salting out” method), and solid-phase extraction, which is the most widely used method today. Solid-phase extraction utilizes the selective binding of nucleic acids to an ion-exchange matrix, silica-based membrane, magnetic silica particles or other chemistries using appropriate buffer conditions.
Considering time and cost, the silica-based membrane is, by far, the most suitable means for small-scale nucleic acid purification. It selectively binds DNA or RNA at different hydrophobic
