Monthly Archives: December 2016
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- 十二月 07, 2016
Cell-based assays are widely used in basic and translational research as cost-effective and accessible models to mimic in vivo responses. To obtain reliable data, assessing the health of cultured cells prior to any assays is essential. Furthermore, many cell-based assays require quantification of cell growth. Cell health and growth can be determined by quantifying cell viability, proliferation, or apoptosis. Below, we compare some commonly used assays to help you determine which type is suitable for your experimental design.
- Cell viability assays enumerate the ratio of live and dead cells in a population. Cell viability can simply be achieved by staining and counting live or dead cells. A deeper assessment of cell health can be attained by measuring cell metabolic activity, such as the ability to reduce tetrazolium salts in MTT and WST-1 assays.
- Cell proliferation assays assess dividing cells. Some common assays include BrdU incorporation (BrdU Assays) that can directly measure DNA synthesis,
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