Monthly Archives: July 2017
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- 七月 05, 2017
Cell culturing is a widely used technique to grow cells outside of their natural environment using artificial environments and controlled conditions that has become indispensable to scientific research. The applications of cell culturing are innumerable and there are many ways to differentiate types of cell culturing. For instance, there are cells isolated from normal or disease models, primary or immortalized, adherent or suspension, 2D or 3D. This blog post will focus on 2D cell culturing versus 3D cell culturing to ostend ScienCell’s new line of 3D culturing kits that model endothelial tube formation.
Traditional 2D culturing generally involves growing adherent cells on a flat plastic surface such as in a T-75 flask or a tissue culturing dish. Depending on the cell type, sometimes the plastic surface is coated with an extracellular matrix component or biological compound to promote cell attachment such as fibronectin or poly-L-lysine. In the case of normal primary cells,