Focusing the Spotlight on Lens Epithelial Cells
The ocular lens is a transparent structure in the eye that is designed to refract and focus light onto the retina. The lens is an avascular unit which includes the… Read more »
Tag Archives: primary cells
Microglia: Front Line Defenders of the Central Nervous System
Microglia play an essential role in brain homeostasis, neuroinflammation, neurodegenerative diseases, and brain infections. Microglia are integral components of the neuro-glial cell network and are the resident immune cells of… Read more »
Not All Primary Cells are Created Equal
Primary cells, which are isolated directly from tissue, show normal cell morphology and maintain many of the important markers and functions seen in vivo. Primary cells, though, have a finite lifespan and limited expansion capacity, so it is critical to use primary cells at low passage for your research.
Primary Neurons vs Neuronal Cell Lines
Neuronal cell lines are commonly used for in vitro neurobiology studies because they are more easily transfected compared to primary neurons and they proliferate, whereas primary neurons do not. Neuronal… Read more »
Circadian Clock and its connection to Breast Cancer
The 2017 Nobel Prize in Physiology or Medicine was awarded to Jeffrey C Hall, Michael Rosbash, and Michael W Young for research that established key mechanistic principles on how circadian… Read more »
How to Take Quality Microscopy Pictures
When it comes to primary cell culture, a picture is worth a thousand words. A quality image can convey information on cell morphology, health, culture purity and density. Different types… Read more »
Choosing the Right Substrate
Many primary cells have difficulty adhering to uncoated glass and plastic surfaces, especially in low serum or serum-free conditions. For primary cells, it is necessary to use a substrate coating… Read more »
The Advantages and Difficulties of Working with Primary Cells
Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical… Read more »
6 common mistakes when working with primary cells
When working with primary cells, it is important to remember that they are not cell lines and cannot be treated the same. Because ScienCell has extensively worked with primary cells, we… Read more »
Primary Cells Versus Cell Lines
Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical… Read more »