Evaluate cAMP-dependent signaling with ScienCell’s CRE/CREB Dual Reporter Vector (CREVR, Cat. No. VT004). CREB regulates gene expression in response to cAMP/PKA, calcium, and other signaling pathways, controlling metabolism, neuronal activity, and stress responses. Accurate measurement of CRE activity is crucial for signaling research, pharmacology, and drug discovery.
The vector integrates a Firefly luciferase under CRE/CREB control together with a constitutively expressed Renilla luciferase for built-in normalization, plus a negative control vector to distinguish pathway-specific activation from background.
Key Benefits:
- Accurate quantification of CRE/CREB pathway activity
- Internal Renilla control improves reproducibility
- Negative control vector ensures trustworthy interpretation
Applications:
Recommended for studies in cAMP signaling, GPCR activation, neuronal research, and drug discovery.
For robust, quantitative results, pair the CRERV (Cat. No. VT004) with:
- UniFectagen Transfection Reagent (Cat. No. 0983)
- Transfection Max Medium (TMM, Cat. No. 9051)
- Luciferase Assay Kit (LAK, Cat. No. 9048)
- Firefly Assay Enhancer (100X) (FAE, Cat. No. 7208)
This setup allows clear distinction of true CRE-mediated activity from background signals.
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