Monthly Archives: November 2017
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- November 30, 2017
Primary cells, which are isolated directly from tissue, show normal cell morphology and
maintain many of the important markers and functions seen in vivo. Primary cells, though, have a finite lifespan and limited expansion capacity, so it is critical to use low passage primary cells for your research.Remember that primary cells are never 100% pure, so if cells at higher passage are used there is greater risk of having contaminating cells outgrow the cells of interest. With each passage, phenotypic and genotypic changes occur as the cells diverge from the original isolated cell population. Additionally, these genetic changes can lead to epigenetic changes. We, therefore, strongly encourage you to use primary cells as early as possible for experiments to prevent genetic drift. Although some primary cell types, such as fibroblasts, can be extremely proliferative and can be passaged many
