Monthly Archives: January 2016
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- January 22, 2016
Cell culture studies provide a valuable complement to in vivo experiments, allowing for a more controlled manipulation of cellular functions and processes. For decades, cell lines have played a critical role in scientific advancements, yet researchers have become increasingly cautious when interpreting data generated from cell lines only. Factors such as misidentified and contaminated cell lines have spurred renewed interest in primary cells [1, 2]. Moreover, cell lines often differ genetically and phenotypically from their tissue origin, whereas primary cells maintain many of the important markers and functions seen in vivo [3, 4]. Endothelial cell lines, for example, lack various functional markers, while primary endothelial cells retain these critical features. Despite these advantages, obtaining a pure population of primary cells can be a difficult and arduous process. At ScienCell Research Laboratories, where we specialize in primary cell culture, we understand the many challenges
