Mesenchymal Stem Cell Medium-serum free
Mesenchymal Stem Cell Medium-serum free (MSCM-sf) consists of 500 ml of basal medium, 5 ml of Mesenchymal Stem Cell Growth Supplement-serum free (MSCGS-sf, Cat. #7562) and 5 ml of penicillin/streptomycin solution (P/S, Cat. #0503).
Mesenchymal Stem Cell Medium-serum free (MSCM-sf), when used with Mesenchymal Stem Cell Growth Supplement-serum free (MSCGS-sf, Cat #7562), is a chemically defined medium designed for optimal growth of normal mesenchymal stem cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mesenchymal stem cells in vitro.
| Catalog No. | 7511 |
|---|---|
| Country of Manufacture | United States |
| Product Code | MSCM-sf |
| Size/Quantity | 500 ml |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store the basal medium at 4° C, the MSCGS-sf and the P/S solution at -20° C. Protect from light. |
| Shipping | Basal medium: ambient temperature. Supplements: dry ice. |
Background
Squamous cell carcinoma of the tongue (TSCC) is an aggressive malignancy with few therapeutic modalities available. Cisplatin, a commonly used chemotherapeuti... More
Background
Squamous cell carcinoma of the tongue (TSCC) is an aggressive malignancy with few therapeutic modalities available. Cisplatin, a commonly used chemotherapeutic drug, is often associated with significant toxicities and resistance. The use of an exosome-enriched fraction of extracellular vesicles (EVs) as a drug delivery system has become an attractive way to potentiate therapeutic effects and decrease toxicity. In particular, our focus is to study the therapeutic effect of cisplatin-loaded EVs obtained from umbilical cord mesenchymal stem cells (UC-MSCs) on TSCC. The goal is to assess the functionality of cancer cells and to characterize the EVs loaded with cisplatin.
Materials and methods
Cisplatin was incorporated into EVs derived from UC-MSCs. Characterization was performed using transmission electron microscopy (TEM) for morphology, BCA assay for protein quantification, and HPLC for drug loading efficiency. The presence of apoptotic bodies was evaluated using cytotoxicity assays and inverted phase-contrast microscopy to assess the effects of EV-encapsulated cisplatin on TSCC cells.
Results
Cisplatin-encapsulated EVs presented a statistically significantly more cytotoxic effect on cancer cells than unencapsulated cisplatin, with the half-maximal inhibitory concentration (IC50) of the EV formulation (1.64 µg/mL) representing a 25% enhancement in potency compared to free cisplatin (2.18 µg/mL; p Less
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the products.
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