大鼠巨噬细胞

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. In the bone marrow and subsequestly in the blood and tissues, monocytes undergo a cascade of maturation that culminate in morphologically and functionally mature tissue macrophages. The main function of macrophages is to remove cellular debris and destroy invading pathogens. They phagocytize invading microorganisms, and scavenge dead, damaged cells and cellular debris. Macrophages can be identified by the specific expression of several cell surface proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by flow cytometry or immunohistochemical staining.

RMa-bm from ScienCell Research Laboratories are isolated from adult rat bone marrow. RMa-bm are harvested at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. RMa-bm are characterized by immunofluorescence with antibody specific to F4/80. RMa-bm are negative for mycoplasma, bacteria, yeast, and fungi. RMa-bm are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RMa-bm are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing RMa-bm in vitro.

巨噬细胞由循环中的骨髓来源单核细胞分化而来。单核细胞在骨髓中形成后，随后进入血液和组织，并经历一系列成熟过程，最终分化为形态和功能均成熟的组织巨噬细胞。巨噬细胞的主要功能是清除细胞碎片并消灭入侵病原体。它们能够吞噬入侵的微生物，并清除死亡或受损细胞及细胞碎片。通过流式细胞术或免疫组织化学染色，可根据细胞表面特异性蛋白的表达对巨噬细胞进行鉴定，包括 CD14、CD11b、F4/80（小鼠）/EMR1（人）、MAC-1/MAC-3 和 CD68。

ScienCell Research Laboratories 提供的 大鼠巨噬细胞（RMa-bm） 分离自成年大鼠骨髓。RMa-bm 在 P0 代收获，并以冷冻状态提供。每瓶含有 >1 × 10⁶ 个细胞，体积为 1 ml。RMa-bm 采用针对 F4/80 的特异性抗体进行免疫荧光表征。RMa-bm 的支原体、细菌、酵母和真菌检测结果均为阴性。根据 ScienCell Research Laboratories 提供的培养条件，RMa-bm 可保证继续进行培养；但由于这些细胞在常规培养条件下不发生增殖，因此不建议用于细胞扩增或长期培养。

推荐培养基

推荐使用巨噬细胞培养基（Macrophage Medium，MaM，产品编号：1921）进行 RMa-bm 的体外培养。

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