CD1 小鼠肝巨噬细胞

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy invading pathogens. Mouse Hepatic Macrophages (MHMa), which are also known as Kupffer cells, reside within the lumen of liver sinusoids. MHMa protect the liver by responding to pathogens and metastatic cells, while tolerating harmless self and foreign antigens, which enter via blood flow through the portal vein and hepatic artery. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, fatty liver disease, and liver transplantation. MHMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.

MHMa from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 CD-1 mouse liver. MHMa are cryopreserved at P0 and delivered frozen. Each vial contains > 1 x 10⁶ cells in 1 ml volume. MHMa are characterized by immunofluorescence with antibody to F4/80. MHMa are negative for mycoplasma, bacteria, yeast, and fungi. MHMa are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, MHMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing MHMa in vitro.

巨噬细胞是由骨髓来源的循环单核细胞分化而来的免疫细胞，其主要功能是清除细胞碎片并吞噬和杀灭侵入机体的病原体。小鼠肝巨噬细胞（MHMa），又称库普弗细胞（Kupffer cells），主要分布于肝窦腔内。MHMa 可通过识别并应对病原体及转移性肿瘤细胞来保护肝脏，同时对经门静脉及肝动脉血流进入的无害自身抗原和外源抗原保持免疫耐受。近年来研究表明，肝巨噬细胞在肝纤维化、肝脏炎症、脂肪性肝病及肝移植等过程中发挥重要作用。MHMa 是研究巨噬细胞在正常生理及病理条件下功能的理想体外模型。

ScienCell Research Laboratories 的小鼠肝巨噬细胞（MHMa）分离自出生后第 2 天或第 8 天 CD-1 小鼠肝脏组织。MHMa 于第 0 代（P0）进行冻存，并以冻存状态交付。每支冻存管含 >1 x 10⁶ 个细胞，总体积为 1 mL。MHMa 经 F4/80 抗体免疫荧光染色鉴定。MHMa 经检测不含支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下可继续培养；但由于该细胞在常规培养条件下不具备增殖能力，因此不建议用于扩增或长期培养。

推荐培养基

建议使用巨噬细胞培养基（Macrophage Medium，MaM，产品编号：1921）进行 MHMa 的体外培养。