大鼠脾脏巨噬细胞

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy invading pathogens. Rat Splenic Macrophages (RSMa) reside in the spleen and play a critical role in maintaining blood homeostasis. There are three distinct populations of splenic macrophages including: red pulp macrophages, marginal zone macrophages, and marginal zone metallophillic macrophages. RSMa perform iron processing functions and aid in the capture of microbes and viruses in the circulatory system. Recent studies have shown that splenic macrophages contribute to stroke pathology and autoimmune diseases. RSMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.

RSMa from ScienCell Research Laboratories are isolated from adult rat spleen. RSMa are cryopreserved at P0 and delivered frozen. Each vial contains > 1 x 10⁶ cells in 1 ml volume. RSMa are characterized by immunofluorescence with antibody to F4/80. RSMa are negative for mycoplasma, bacteria, yeast, and fungi. RSMa are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, RSMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing RSMa in vitro.

巨噬细胞由来源于骨髓的循环单核细胞分化而来，其主要功能是清除细胞碎片并吞噬、杀灭入侵的病原体。大鼠脾脏巨噬细胞（RSMa）主要分布于脾脏，在维持血液稳态中发挥重要作用。脾脏中存在三种不同类型的巨噬细胞，包括红髓巨噬细胞、边缘区巨噬细胞以及边缘区金属亲和性巨噬细胞。RSMa参与铁代谢过程，并有助于捕获循环系统中的微生物和病毒。近期研究表明，脾脏巨噬细胞还参与脑卒中及自身免疫性疾病的发生发展。因此，RSMa是研究巨噬细胞在正常生理及病理状态下功能机制的理想模型。

ScienCell Research Laboratories 提供的RSMa来源于成年大鼠脾脏组织分离获得，在P0代进行冻存保存，并以冷冻状态交付。每支冻存管含有超过1×10⁶个细胞，体积为1 ml。该细胞经免疫荧光检测确认表达F4/80标志物，同时支原体、细菌、酵母及真菌检测均为阴性。RSMa可在ScienCell提供的培养条件下继续短期培养，但不建议进行扩增或长期传代培养，因为该细胞在常规培养条件下不具备增殖能力。

推荐培养基

建议使用巨噬细胞培养基（MaM，货号#1921）进行RSMa的体外培养。