人肝巨噬细胞

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy invading pathogens. Human Hepatic Macrophages (HHMa), which are also known as Kupffer cells, reside within the lumen of liver sinusoids. HHMa protect the liver by responding to pathogens and metastatic cells, while tolerating harmless self and foreign antigens, which enter via blood flow through the portal vein and hepatic artery. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, fatty liver disease, and liver transplantation. HHMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.

HHMa from ScienCell Research Laboratories are isolated from human liver. HHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 10⁶ cells in 1 ml volume. HHMa are characterized by immunofluorescence with antibody to F4/80. HHMa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHMa are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, HHMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing HHMa in vitro

巨噬细胞由循环于血液中的骨髓来源单核细胞分化而来。巨噬细胞的主要功能是清除细胞碎片并消灭入侵的病原体。人肝巨噬细胞（HHMa），又称库普弗细胞（Kupffer细胞），存在于肝窦腔内。HHMa通过响应病原体和转移性细胞，同时对经由门静脉和肝动脉随血流进入肝脏的无害自身抗原和外来抗原保持耐受，从而保护肝脏。近期研究表明，肝巨噬细胞在肝纤维化、肝脏炎症、脂肪肝疾病和肝移植中发挥重要作用。HHMa是研究正常生理和病理条件下巨噬细胞功能的理想模型。

ScienCell Research Laboratories提供的HHMa分离自人肝脏。HHMa在纯化后进行冻存，并以冷冻状态运输。每支冻存管含有>1 × 10⁶个细胞，体积为1 ml。HHMa采用F4/80抗体进行免疫荧光鉴定。HHMa经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。根据ScienCell Research Laboratories提供的培养条件，HHMa可进一步进行培养；但不建议用于细胞扩增或长期培养，因为HHMa在常规培养条件下不会增殖。

推荐培养基
建议使用巨噬细胞培养基（MaM，产品编号1921）对HHMa进行体外培养。

Search for a Certificate of Analysis by Lot Number below:

No Publication available at this time.