Macrophages are cells differentiated from circulating bone marrow-derived monocytes. In the bone marrow and subsequestly in the blood and tissues, monocytes undergo a cascade of maturation that culminate in morphologically and functionally mature tissue macrophages. The main function of macrophages is to remove cellular debris and destroy invading pathogens. They phagocytize invading microorganisms, and scavenge dead, damaged cells and cellular debris. Macrophages can be identified by the specific expression of several cell surface proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by flow cytometry or immunohistochemical staining.
MMa-bm from ScienCell Research Laboratories are isolated from adult CD-1 mouse bone marrow. MMa-bm are harvested at P0 and delivered frozen. MMa-bm are characterized by immunofluorescence with antibody specific to F4/80 and/or CD11b. MMa-bm are negative for mycoplasma, bacteria, yeast, and fungi. MMa-bm are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MMa-bm are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.
Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing MMa-bm in vitro.
巨噬细胞是由循环中的骨髓来源单核细胞分化而来的细胞。在骨髓中以及随后进入血液和组织后，单核细胞经历一系列成熟过程，最终形成形态和功能均成熟的组织巨噬细胞。巨噬细胞的主要功能是清除细胞碎片并消灭入侵的病原体。它们通过吞噬作用清除入侵的微生物，并清除死亡或受损的细胞及细胞碎片。巨噬细胞可通过流式细胞术或免疫组织化学染色，根据其特异性表达的多种细胞表面蛋白进行鉴定，包括CD14、CD11b、F4/80（小鼠）/EMR1（人）、MAC-1/MAC-3和CD68。
ScienCell Research Laboratories提供的骨髓来源小鼠巨噬细胞（MMa-bm）分离自成年CD-1小鼠骨髓。MMa-bm在P0代收获，并以冷冻状态运输。MMa-bm采用免疫荧光法进行鉴定，使用针对F4/80和/或CD11b的特异性抗体进行检测。MMa-bm经检测不含支原体、细菌、酵母和真菌。在ScienCell Research Laboratories提供的培养条件下，MMa-bm可进一步进行培养；但是，由于MMa-bm在常规培养条件下不具有增殖能力，因此不建议用于细胞扩增或长期培养。
推荐培养基
建议使用巨噬细胞培养基（Macrophage Medium，MaM，产品编号1921）对MMa-bm进行体外培养。
| 目录编号 | M1920 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | MMa-bm |
| 规格/数量 | 1 x 10^6 cells/vial, 2 x 10^6 cells/vial, 5 x 10^6 cells/vial, or 10 x 10^7 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰中直接转移至液氮中，并在实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
Product Sheets
M1920-2 (2 Million Cells per Vial)
M1920-5 (5 Million Cells per Vial)
M1920-10 (10 Million Cells per Vial)
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