Recombinant Human Wnt-3a Protein, E. coli Derived
Recombinant Human Wnt-3a Protein, E. coli Derived (rhWnt3AE) is expressed in E. coli, engineered with an N-terminal His-tag for ease of purification and detection. It is ideal for use in developmental biology research, and Wnt signaling studies.
Wnt3a is one of 19 vertebrate members of the highly conserved Wingless-type MMTV integration site (Wnt) family. It is secreted cysteine-rich glycoproteins and critical for embryonic development and adult tissue homeostasis. Wnt3a functions as a key ligand in the canonical Wnt signaling pathway, binding Frizzled receptors in conjunction with LRP5/6 co-receptors, leading to the stabilization of cytoplasmic β-catenin. Stabilized β-catenin translocate to the nucleus, where it associates with TCF/LEF transcription factors to regulate expression of Wnt target genes. Wnt3a plays a pivotal role in embryonic mesoderm development, caudal somite patterning, neural tube morphogenesis, and the self-renewal of stem cells in the intestinal crypt. It also supports the maintenance of various stem cell populations, including hematopoietic, neural, and embryonic stem cells. This Recombinant Human Wnt3a is expressed in E. coli, engineered with an N-terminal His-tag for ease of purification and detection. It is ideal for use in developmental biology research, and Wnt signaling studies.
| Catalog No. | 109-03AE |
|---|---|
| Country of Manufacture | United States |
| Product Code | rhWnt3AE |
| Size/Quantity | 10 ug, 100 ug and 1 mg |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receipt, store at -20°C after receiving. Upon reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. |
| Shipping | Gel pack |
| Synonyms | Wnt-3a, Wingless-type MMTV integration site family member 3A |
| AA Sequence | MGSSHHHHHH SSGLVPRGSH MYPIWWSLAV GPQYSSLGSQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GIKIGIQECQ HQFRGRRWNC TTVHDSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGTAAICGC SSRHQGSPGK GWKWGGCSED IEFGGMVSRE FADARENRPD ARSAMNRHNN EAGRQAIASH MHLKCKCHGL SGSCEVKTCW WSQPDFRAIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRP RYTYFKVPTE RDLVYYEASP NFCEPNPETG SFGTRDRTCN VSSHGIDGCD LLCCGRGHNA RAERRREKCR CVFHWCCYVS CQECTRVYDV HTCK |
| Source | E. coli |
| Molecular Weight | 40.1 kDa, non-glycosylated polypeptide chains containing 352 amino acids |
| Purity | > 90% |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2 ÎĽm filtered concentrated (1mg/ml) solution in 40 mM HCl. |
| Endotoxin | <0.1 ng/ÎĽg of protein (<1 EU/ÎĽg) |
| Reconstitution | Reconstitute in sterile distilled water or 20 mM HCl to a concentration of 0.1-1.0 mg/mL. |
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