Recombinant Human Wnt-3a Protein Fragment 19-87, 294-352
Recombinant Human Wnt-3a Protein Fragment 19-87, 294-352 (rhWnt3AEF12) is a recombinant peptide corresponding to human Wnt3a protein fragment (AA 19-87, 294-352).
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Wnt3a is one of 19 vertebrate members of the highly conserved Wingless-type MMTV integration site (Wnt) family. It is secreted cysteine-rich glycoproteins and critical for embryonic development and adult tissue homeostasis. Wnt3a functions as a key ligand in the canonical Wnt signaling pathway, binding Frizzled receptors in conjunction with LRP5/6 co-receptors, leading to the stabilization of cytoplasmic β-catenin. Stabilized β-catenin translocate to the nucleus, where it associates with TCF/LEF transcription factors to regulate expression of Wnt target genes. Wnt3a plays a pivotal role in embryonic mesoderm development, caudal somite patterning, neural tube morphogenesis, and the self-renewal of stem cells in the intestinal crypt. It also supports the maintenance of various stem cell populations, including hematopoietic, neural, and embryonic stem cells. This product is a recombinant peptide corresponding to human Wnt3a protein fragment (AA 19-87, 294-352). This region is located within Wnt3a and contains conserved amino acid residues that may contribute to protein folding and biological activity.
| Catalog No. | 109-03AEF12 |
|---|---|
| Country of Manufacture | United States |
| Product Code | rhWnt3AEF12 |
| Size/Quantity | 10ug, 100ug |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receipt, store at -20°C after receiving. Upon reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. |
| Shipping | Gel pack |
| Synonyms | Wnt-3a, Wingless-type MMTV integration site family member 3A |
| AA Sequence | SYPIWWSLAV GPQYSSLGSQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GIKIGIQECQ FRGRRWNDRT CNVSSHGIDG CDLLCCGRGH NARAERRREK CRCVFHWCCY VSCQECTRVY DVHTCK |
| Source | E. coli |
| Molecular Weight | 14.4 kDa, non-glycosylated polypeptide chains containing 126 amino acids |
| Purity | > 90% |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 1x PBS |
| Endotoxin | <0.1 ng/μg of protein (<1 EU/μg) |
| Reconstitution | Reconstitute in sterile distilled water or 1x PBS to a concentration of 0.1-1.0 mg/mL |
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