Recombinant Human Wnt-3a Protein Fragment 19-87
Recombinant Human Wnt-3a Protein Fragment 19-87 (rhWnt3AEF2) is a recombinant peptide corresponding to human Wnt3a protein fragment (AA 19-87, indicated with underlining), fused with a tag to facilitate expression and purification.
Customized sizes available. Please inquire.
Wnt3a is one of 19 vertebrate members of the highly conserved Wingless-type MMTV integration site (Wnt) family. It is secreted cysteine-rich glycoproteins and critical for embryonic development and adult tissue homeostasis. Wnt3a functions as a key ligand in the canonical Wnt signaling pathway, binding Frizzled receptors in conjunction with LRP5/6 co-receptors, leading to the stabilization of cytoplasmic β-catenin. Stabilized β-catenin translocate to the nucleus, where it associates with TCF/LEF transcription factors to regulate expression of Wnt target genes. Wnt3a plays a pivotal role in embryonic mesoderm development, caudal somite patterning, neural tube morphogenesis, and the self-renewal of stem cells in the intestinal crypt. It also supports the maintenance of various stem cell populations, including hematopoietic, neural, and embryonic stem cells. This product is a recombinant peptide corresponding to human Wnt3a protein fragment (AA 19-87, indicated with underlining), fused with a tag to facilitate expression and purification. This region is located within the mid-to-C-terminal domain of Wnt3a and contains conserved amino acid residues that may contribute to protein folding and biological activity.
| Catalog No. | 109-03AEF2 |
|---|---|
| Country of Manufacture | United States |
| Product Code | rhWnt3AEF2 |
| Size/Quantity | 10ug, 100ug |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receipt, store at -20°C after receiving. Upon reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. |
| Shipping | Gel pack |
| Synonyms | Wnt-3a, Wingless-type MMTV integration site family member 3A |
| AA Sequence | MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM GSSHHHHHHS SGLVPRGSHM ASMSDSEVNQ EAKPEVKPEV KPETHINLKV SDGSSEIFFK IKKTTPLRRL MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG SYPIWWSLAV GPQYSSLGSQ PILCASIPGL VPKQLRFCRN YVEIMPSVAE GIKIGIQECQ HQFRGRRWN |
| Source | E. coli |
| Molecular Weight | 38.7 kDa, non-glycosylated polypeptide chains containing 349 amino acids |
| Purity | > 90% |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 1x PBS. |
| Endotoxin | <0.1 ng/μg of protein (<1 EU/μg) |
| Reconstitution | Reconstitute in sterile distilled water or 1x PBS to a concentration of 0.1-1.0 mg/mL |
No Publication available at this time.
