Mesenchymal Stem Cell Medium-serum free (MSCM-sf), when used with Mesenchymal Stem Cell Growth Supplement-serum free (MSCGS-sf, Cat #7562), is a chemically defined medium designed for optimal growth of normal mesenchymal stem cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mesenchymal stem cells in vitro.