C57BL/6小鼠真皮成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.

MDF from ScienCell Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse skin. MDF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MDF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. MDF are negative for mycoplasma, bacteria, yeast, and fungi. MDF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories. 

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MDF in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于其是最容易进行体外培养的细胞类型之一，因此已被广泛用于各种细胞与分子研究。其良好的耐受性也使其适用于多种实验操作，包括基因转染研究以及显微注射等。通常情况下，成纤维细胞会分泌富含I型和/或III型胶原蛋白的非刚性细胞外基质。有证据表明，不同器官来源的成纤维细胞在本质上存在差异。真皮成纤维细胞在伤口愈合过程中会从增殖与迁移阶段转变为收缩及基质重塑阶段。此外，它们在炎症刺激下还会分泌大量透明质酸。

ScienCell Research Laboratories 提供的MDF分离自出生后第2天（postnatal day 2）的C57BL/6小鼠皮肤。MDF在第一代（passage one）时进行冷冻保存，并以冻存形式交付。每瓶含有超过5 × 10⁵个细胞，总体积为1 ml。MDF通过梭形成纤维样形态以及针对纤维连接蛋白（fibronectin）的特异性抗体免疫荧光进行鉴定。MDF经检测不含支原体、细菌、酵母及真菌。在ScienCell Research Laboratories提供的培养条件下，保证MDF可进一步扩增5次群体倍增（population doublings）。 

推荐培养基

建议使用 Fibroblast Medium-2（FM-2，产品编号 #2331）用于MDF的 in vitro 培养。