成纤维细胞培养基-2

Fibroblast Medium-2 (FM-2), when used with Fibroblast Growth Supplement-2 (FGS-2, Cat #2382) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal fibroblasts in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal fibroblasts in vitro.

成纤维细胞培养基-2（FM-2）与成纤维细胞生长补充剂-2（FGS-2，产品编号 2382）和25 mL胎牛血清（FBS）配合使用时，可组成一种专为正常成纤维细胞体外最佳生长而设计的完全培养基。该培养基为无菌液体培养基，含有必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质以及低浓度胎牛血清（5%）。培养基采用HEPES和碳酸氢盐缓冲体系，在5% CO₂/95%空气条件下平衡后，pH值为7.4。该培养基经过定量和定性优化配制，可提供均衡的营养环境，选择性支持正常成纤维细胞的体外生长。