成年大鼠真皮成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.

RDF-a from ScienCell Research Laboratories are isolated from adult rat skin. RDF-a are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RDF-a are characterized by immunofluorescence with antibody specific to fibronectin. RDF-a are negative for mycoplasma, bacteria, yeast, and fungi. RDF-a are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended medium:

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing RDF-a in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于成纤维细胞是最容易进行体外培养的细胞类型之一，因此被广泛用于各种细胞和分子研究。其良好的耐受性也使其适用于多种实验操作，包括基因转染和显微注射等研究。通常情况下，成纤维细胞分泌一种非刚性的细胞外基质，其中富含I型和/或III型胶原蛋白。有证据表明，不同器官中的成纤维细胞在本质上存在差异。皮肤伤口愈合过程中，真皮成纤维细胞会从增殖、迁移阶段转变为收缩、基质重塑阶段。此外，在炎症刺激下，它们会分泌大量透明质酸。

ScienCell Research Laboratories提供的成年大鼠真皮成纤维细胞（RDF-a）分离自成年大鼠皮肤。RDF-a在第1代进行冻存，并以冷冻状态运输。每瓶含有超过5 × 10⁵个细胞，体积为1 ml。RDF-a采用免疫荧光法进行鉴定，使用针对纤连蛋白的特异性抗体进行检测。RDF-a经检测不含支原体、细菌、酵母和真菌。在ScienCell Research Laboratories提供的培养条件下，RDF-a可保证进一步扩增5个群体倍增。

推荐培养基：

建议使用成纤维细胞培养基-2（Fibroblast Medium-2，FM-2，产品编号2331）对RDF-a进行体外培养。

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