牛皮肤成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. Dermal fibroblasts have the ability to switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. After injury, integrins, extracellular matrix, and cytokines can modulate fibroblast function in order to stimulate tissue repair. Cultured Bovine Dermal Fibroblasts (BDF) are a useful in vitro model to study the mechanisms of wound healing and to develop therapeutic targets to reduce scarring and fibrosis.

BDF from ScienCell Research Laboratories are isolated from fetal bovine skin. BDF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. BDF are characterized by immunofluorescence with antibody specific to fibronectin. BDF are negative for mycoplasma, bacteria, yeast, and fungi. BDF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing BDF in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于其是最容易进行体外培养的细胞类型之一，因此已被广泛应用于各种细胞与分子研究。其良好的耐受性也使其适用于多种实验操作，包括基因转染研究和显微注射等。通常情况下，成纤维细胞会分泌富含I型和/或III型胶原蛋白的非刚性细胞外基质。在伤口愈合过程中，皮肤成纤维细胞能够从增殖和迁移阶段转变为收缩及基质重塑阶段。损伤发生后，整合素、细胞外基质以及细胞因子可调节成纤维细胞功能，从而促进组织修复。培养的牛皮肤成纤维细胞（BDF）是研究伤口愈合机制以及开发减少瘢痕与纤维化治疗靶点的有用体外模型。

ScienCell Research Laboratories 提供的BDF分离自胎牛皮肤。BDF在第一代（passage one）时进行冷冻保存，并以冻存形式交付。每瓶含有超过 5 x 10^5 个细胞，总体积为1 ml。BDF经针对纤维连接蛋白（fibronectin）的特异性抗体免疫荧光鉴定。BDF经检测不含支原体、细菌、酵母及真菌。在ScienCell Research Laboratories提供的培养条件下，保证BDF可进一步扩增5次群体倍增（population doublings）。

推荐培养基

推荐使用 Fibroblast Medium-2（FM-2，货号 #2331）用于BDF的 in vitro 培养。