Category Archives: Gene Expression Profiling

breast_cancer_sciencell_blog

Circadian Clock and its connection to Breast Cancer

The 2017 Nobel Prize in Physiology or Medicine was awarded to Jeffrey C Hall, Michael Rosbash, and Michael W Young for research that established key mechanistic principles on how circadian… Read more »

Value of qPCR in the Next Generation Sequencing Era

qPCR is still considered the gold standard for gene expression analysis, especially when template copy number is low. In short, NGS provides a great tool for analyzing a large number of genes to narrow down the research interest and qPCR provides a fast, reliable, and cost effective means to verify NGS results.

pseudogene timeline

Hidden Treasure in “Junk DNA”

3 Reasons Why You Should Take “Pseudogenes” Seriously in Your Research Protein-coding DNA, or “functional” genes, only accounts for up to 2% in the human genome. Until recently, the remaining… Read more »

Perspectives on Cancer

Perspectives on Cancer

Cancer is a collection of over 200 diseases where the only common denominator is rogue cells1,2.  The ways in which a cell can go rogue is so varied that cancer… Read more »

Comparison of Cell Viability, Proliferation and Apoptosis Assays

To obtain reliable data, assessing the health of cultured cells prior to any assays is essential. Furthermore, many cell-based assays require quantification of cell growth. Cell health and growth can be determined by quantifying cell viability, proliferation, or apoptosis