qPCR is a powerful tool for quantification of gene expression levels and copy number variation. Despite the advances in next-generation sequencing (NGS), qPCR still serves as the “gold standard” for… Read more »
Cell-based assays are widely used in basic and translational research as cost-effective and accessible models to mimic in vivo responses. To obtain reliable data, assessing the health of cultured cells prior to… Read more »
When working with primary cells, it is important to remember that they are not cell lines and should be treated with care. At ScienCell, we specialize in primary cell culture… Read more »
Cancer immunotherapy is one promising cancer treatment option whereby the host’s own immune system is used to treat cancer. The therapy works by either stimulating certain immune activities, or counteracting… Read more »
Aging and Telomere Length Quantification by qPCR Aging is a time-dependent decline of the body’s functional capabilities and is an inevitable course of life (as shown in the image below,… Read more »
Primary cells, which are isolated directly from tissue, show normal cell morphology and maintain many of the important markers and functions seen in vivo. Primary cells, though, have a finite lifespan and limited expansion capacity, so it is critical to use primary cells at low passage for your research.
Neuronal cell lines are commonly used for in vitro neurobiology studies because they are more easily transfected compared to primary neurons and they proliferate, whereas primary neurons do not. Neuronal… Read more »
The 2017 Nobel Prize in Physiology or Medicine was awarded to Jeffrey C Hall, Michael Rosbash, and Michael W Young for research that established key mechanistic principles on how circadian… Read more »
qPCR is still considered the gold standard for gene expression analysis, especially when template copy number is low. In short, NGS provides a great tool for analyzing a large number of genes to narrow down the research interest and qPCR provides a fast, reliable, and cost effective means to verify NGS results.