Why Every Researcher Should Care About Metabolite Assays?

Several key metabolites play crucial roles in cellular bioenergetic homeostasis. ScienCell has developed sensitive assays to accurately quantify L-lactate, glucose, glutamate, and pyruvate. 

L-Lactate is a key intermediate in glucose metabolism. Under hypoxic conditions, lactate dehydrogenase (LDH) converts pyruvate, the final product of glycolysis, into L-lactate. Monitoring lactate levels provides valuable insights into tissue oxygen balance and overall cellular physiology.  

Our L-Lactate Assay (Cat. No. 8308) Kit enables researchers to measure L-lactate levels in various biological samples, including cells, serum, plasma, and tissue extracts. Available in both colorimetric and fluorometric detection formats, the kit offers: 

• Fluorometric detection (Cat. No. 8788): Measurement range from 1 µM to 50 µM L-lactate.
• Colorimetric detection (Cat. No. 8308): Measurement range from 60 µM to 2000 µM. 

Glucose is a vital energy source and key biomarker for metabolic disorders like diabetes, cancer, and Alzheimer’s disease. Our Glucose Assay Kit (Cat. No. 8418) provides a simple, reliable colorimetric method for glucose measurement. The assay utilizes glucose oxidase to generate hydrogen peroxide, which reacts with 4-aminoantipyrine, producing a color change measured at 570 nm. The intensity is directly proportional to glucose concentration, ensuring accurate results for diagnostic and research applications.  

Pyruvate is a key metabolic intermediate, linking glycolysis and the TCA cycle. Its measurement is crucial for studying cellular energy production, mitochondrial function, and metabolic disorders like diabetes and cancer. The ScienCell's Pyruvate Assay (Cat. No. 8388) offers a simple, reliable colorimetric method to quantify pyruvate. Using pyruvate oxidase, the reaction produces a colored dye measured at 570 nm, with intensity directly proportional to pyruvate concentration. This assay delivers accurate, reproducible results, making it an essential tool for metabolic research. 

Glutamate is a key neurotransmitter and metabolic signaling intermediary whose dysfunction is implicated in neurological diseases. The colorimetric Glutamate Assay (Cat. No. 8338) measures glutamate levels in cells, serum, plasma, tissue and food extracts. In this assay, glutamate is oxidized by glutamate dehydrogenase, and the formed NADH reduces a tetrazolium salt (MTT). The resulting-colored product has an absorbance maximum at 570 nm, with its intensity directly proportional to the glutamate concentration. 

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