大鼠心肌细胞
分离自出生后第2天或第8天的 CD® IGS 大鼠心脏。大鼠心肌细胞（RCM） 在 P0 代进行冻存，并以冷冻状态提供。每瓶含有 >1 × 106 个细胞，体积为 1 ml。
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来源于 CD1 小鼠的心肌细胞（M6200）
Cardiac myocytes are the most physically energetic cells in the body. They are highly specialized high-oxygen-content cells that house a large number of mitochondria. They occupy as much as 75% of the cardiac mass, but constitute only about one third of the total cell number in the heart. Differentiated cardiac myocytes have little capacity to proliferate; however, hypertrophic growth has been shown to respond to alpha1-adrenergic stimuli via the Ras/MEK pathway. All cardiac myocytes are capable of spontaneous rhythmic depolarization and repolarization of their membranes. Contraction of cardiac myocytes is myogenic, which is independent of nervous stimulation. There is a complex network of signals in cardiac myocytes regulating the rhythmic pumping of the heart. Cardiac myocyte hypertrophy and apoptosis have been implicated in the loss of contractile function during heart failure. A better understanding of the cardiac signaling network will help reveal the cellular mechanisms leading to cardiac myocyte death.
RCM from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 CD® IGS rat heart. RCM are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. RCM are characterized by immunofluorescence with antibodies specific to sarcomeric alpha-actinin and/or α-smooth muscle actin. RCM are negative for mycoplasma, bacteria, yeast, and fungi. RCM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RCM are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Cardiac Myocyte Medium-serum free (CMM-sf, Cat. #6101) for culturing RCM in vitro.
心肌细胞是机体内代谢活动最为活跃的细胞之一。它们属于高度特化的高耗氧细胞，含有大量线粒体。心肌细胞约占心脏组织质量的 75%，但仅约占心脏细胞总数的三分之一。分化成熟的心肌细胞增殖能力有限；然而，研究表明，其肥大性生长可通过 Ras/MEK 通路响应 α1-肾上腺素能刺激。所有心肌细胞均具有自发性节律性膜去极化和复极化的能力。心肌细胞的收缩具有肌源性，与神经刺激无关。心肌细胞内部存在复杂的信号网络，共同调控心脏的节律性泵血功能。心肌细胞肥大和凋亡与心力衰竭过程中收缩功能的丧失有关。进一步了解心肌细胞中的信号调控网络，有助于揭示导致心肌细胞死亡的细胞机制。
ScienCell Research Laboratories 提供的 RCM 分离自出生后第 2 天或第 8 天的 CD® IGS 大鼠心脏。大鼠心肌细胞（RCM） 在 P0 代进行冻存，并以冷冻状态提供。每瓶含有 >1 × 106 个细胞，体积为 1 ml。RCM 采用针对肌节 α-辅肌动蛋白（sarcomeric alpha-actinin）和/或 α-平滑肌肌动蛋白（α-smooth muscle actin）的特异性抗体进行免疫荧光表征。RCM 的支原体、细菌、酵母和真菌检测结果均为阴性。根据 ScienCell Research Laboratories 提供的培养条件，RCM 可保证继续进行体外培养；但由于这些细胞在培养过程中不发生增殖，因此不建议用于细胞扩增或长期培养。
推荐培养基
推荐使用无血清心肌细胞培养基（Cardiac Myocyte Medium-serum free，CMM-sf，产品编号：6101）进行 RCM 的体外培养。
| 目录编号 | R6200 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | RCM |
| 规格/数量 | 1 x 10^6 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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