ClearCut DNase
ClearCut™ DNase is a recombinant enzyme that selectively degrades contaminating double-stranded DNA in RNA samples without affecting RNA, enabling clean RNA preparations for downstream molecular biology applications.
ClearCut™ DNase is a recombinant double-stranded DNA-specific endonuclease designed for the rapid removal of contaminating DNA from RNA preparations. The enzyme selectively cleaves phosphodiester bonds within double-stranded DNA, generating short oligonucleotide fragments while preserving RNA and other singlestranded nucleic acids.
Its high specificity for double-stranded DNA enables efficient removal of contaminating DNA, including genomic DNA, PCR products, and plasmid DNA, without compromising RNA integrity. This feature makes ClearCut™ DNase particularly suitable for RNA purification workflows and downstream applications where residual DNA may interfere with assay sensitivity and data accuracy.
ClearCut™ DNase can be readily inactivated by incubation at 60°C in the presence of DTT or other reducing agents, eliminating the need for additional cleanup procedures. The enzyme is ideal for use prior to reverse transcription, RT-PCR, RT-qPCR, RNA sequencing, and other RNA-based molecular biology applications.
| 目录编号 | MB6318 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | ccDNase |
| 产品用途 | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| 储存 | Upon receipt, store ClearCut DNase (MB6318a) at -20°C in a manual defrost freezer. Store 10x DNase Enzyme Buffer (Cat. No. MB6038b) at 4°C, or alternatively store it at −20°C together with ClearCut™ DNase. |
| 运输 | Dry ice. |