3D血管生成实验

Angiogenesis is the intricate process of the formation of new capillaries from a pre-existing vascular network. Angiogenesis occurs physiologically in situations such as wound healing and muscle growth. It also plays a role in pathophysiological conditions such as in cancer progression. In cancer, a pro-angiogenic state is triggered, resulting in malignant tumor progression. The new vessel formation supports the propagation of the growing tumor by delivering nutrients and oxygen, while at the same time facilitating the removal of metabolic wastes. In ischemic heart disease, angiogenesis has been perceived as a potential therapeutic approach for treating heart failure arising from blood flow deprivation. Given its significance in various conditions, proper tools to study angiogenesis is crucial. ScienCell has developed a ready-to-use 3D-Angiogenesis Assay (3D-AA) that utilizes endothelial cell spheroids that can be readily embedded into the included collagen matrix. Upon successful stimulation, endothelial cells will begin to invade into its surroundings, mimicking the angiogenic process in vivo. The better physiological reflection of angiogenesis serves as an advantage of the 3D-AA over other in vitro assays such as the tube formation assay. 3D-AA is an excellent assay to conveniently and rapidly screen test compounds for their pro-angiogenic and anti-angiogenic effects. Within 24 to 48 hours, the number and length of sprouts formed can be quantified and utilized as a measure of the pro- or anti-angiogenic effect of the test substance.