I 型胶原蛋白三维凝胶制备试剂盒

Type I collagen, a fibrous protein abundant in connective tissues including tendon, ligament, dermis and blood vessel, is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells and etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function. The ScienCell collagen I-3D Gelling kit includes collagen I purified from rat tail tendon by a modification of the method of Bell et al1 and supplied as a sterile liquid in 1/1000 acetic acid. It also includes a 10X Gelling Buffer, which can be used to adjust the pH and ionic strength of the collagen I solution to allow for the formation of a homogenous gel.

I 型胶原蛋白是一种纤维状蛋白质，广泛存在于肌腱、韧带、真皮和血管等结缔组织中，是细胞外基质（ECM）的主要组成成分，也是决定其抗拉强度的关键因素。I 型胶原蛋白常被用作组织培养表面的薄层涂层，以促进多种细胞的贴附与增殖，包括内皮细胞、成纤维细胞、肝细胞和上皮细胞等。此外，I 型胶原蛋白还可在体外自组装形成三维超分子凝胶，因此是理想的生物支架，有助于细胞呈现更接近体内环境的形态与功能。

ScienCell I 型胶原蛋白三维凝胶制备试剂盒包含采用 Bell 等人¹的方法改良后，从大鼠尾腱中纯化获得的 I 型胶原蛋白，并以无菌液体形式提供，溶于 1/1000 浓度的乙酸溶液中。试剂盒还包含 10X 凝胶缓冲液，可用于调节 I 型胶原蛋白溶液的 pH 值和离子强度，从而促进形成均匀的凝胶。

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