Serum in Cell Culture Today
Scientists are trying to reduce or replace animal serum in culture media due to ethical concerns, rising costs, batch variability, and concerns over infectious agents…
Author Archives: Bonnie Marsick
Primary Neurons vs Neuronal Cell Lines
Neuronal cell lines are commonly used for in vitro neurobiology studies because they are more easily transfected compared to primary neurons and they proliferate, whereas primary neurons do not. Neuronal… Read more »
How to Take Quality Microscopy Pictures
When it comes to primary cell culture, a picture is worth a thousand words. A quality image can convey information on cell morphology, health, culture purity and density. Different types… Read more »
Choosing the Right Substrate
Many primary cells have difficulty adhering to uncoated glass and plastic surfaces, especially in low serum or serum-free conditions. For primary cells, it is necessary to use a substrate coating… Read more »
Why is basal media and supplementation important in primary cell culture?
Choosing which media to use with your primary cells can be daunting. Whether you are replicating a formula from a publication, optimizing your own supplemented classical or nutrient media, or… Read more »
Alzheimer’s Disease
Alzheimer’s disease (AD) affects almost 44 million people worldwide. The disease typically begins with memory difficulties followed by trouble thinking, reasoning and processing emotions. The cause of AD is not… Read more »
Classical Media vs Specialty Media
For decades, researchers have been culturing cells to study normal and diseased biological processes. Many of these studies use immortalized cell lines which proliferate indefinitely in culture. Immortalized cells thrive… Read more »