Human Telomeric repeat–containing RNA detection qPCR Kit

Telomeric repeat–containing RNA (TERRA) is a long noncoding RNA transcribed from chromosome ends that plays a key role in telomere biology. TERRA molecules regulate telomerase activity, coordinate homologous recombination, influence telomere-associated protein complexes, and help maintain chromosome end integrity. Due to their low abundance and similarity to telomeric DNA, reliable detection of TERRA has historically been technically challenging.

The ScienCell Human Telomeric repeat–containing RNA detection qPCR Kit offers a sensitive and specific method for quantifying TERRA transcripts by RT-qPCR. The kit employs a proprietary DNA linker strategy during reverse transcription, which prevents amplification of contaminating genomic DNA during the qPCR step. This eliminates the need for harsh DNA removal procedures that can degrade TERRA and reduce assay accuracy. In the qPCR step, chromosome-specific subtelomeric forward primers are paired with linker–telomeric repeat reverse primers, enabling precise measurement of TERRA levels at individual chromosome ends. The GAPDH control is also included, with GAPDH specific forward and linker–GAPDH reverse primers, to serve as a housekeeping gene for normalization of gene expression analysis.