Human TERT DNA Copy Number Quantification qPCR Assay Kit

Telomerase reverse transcriptase (TERT) is a vital enzyme that maintains telomere length, crucial for cellular immortality and genome stability. The human TERT gene (hTERT) copy number often varies in cancer cells, serving as a key marker in oncogenesis studies. Increased hTERT copy number is linked to cancer development, tumor progression, poor prognosis, and genomic instability by enhancing telomerase activity. During cell immortalization, hTERT integration into the genome is common, making hTERT copy number measurement essential for distinguishing normal cells from immortalized ones.

ScienCell’s Human TERT Copy Number Quantification qPCR Assay Kit (HTCQ) delivers a robust and accurate method to quantify hTERT copy number, driving insights into cancer development. The kit detects hTERT copy number in cell lines immortalized via hTERT overexpression and validates their consistency. The hTERT primer set amplifies a 100 bp-long region of the human TERT gene on chromosome 5. The single copy reference (SCR) primer set targets a 100 bp-long region on human chromosome 17. The reference DNA sample consists of a 1:1 ratio of hTERT copies to SCR copies and serves as a reference for calculating the ratio of hTERT to SCR of target samples.