Human ELOVL2 Promoter and Global DNA Dual Methylation Quantification qPCR Assay Kit

DNA methylation at CpG islands in promoters regulates gene expression. It is a dynamic epigenetic process affected by various genetic and environmental factors. Numerous diseases including cancer have been linked to aberrant global changes in genomic DNA methylation. It is generally thought that global genomic DNA methylation level can be represented by the methylation level at repetitive elements. One example of this includes the repetitive Long Interspersed Nuclear Element-1 (LINE-1), which compromises 17-18% of the human genome and is usually heavily methylated in most normal primary cells. Furthermore, the CpG island methylation level at human ELOVL2 gene promoter has been well-associated with chronological age in various populations, cell types, and tissues. ELOVL2 promoter methylation level is therefore a potential marker for human chronological age. ScienCell's Human ELOVL2 Promoter and Global DNA Dual Methylation Quantification qPCR Assay Kit (HEGDD) is designed to simultaneously quantify the level of human ELOVL2 promoter and LINE1 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated ELOVL2 promoter copies to non-methylated ELOVL2 promotor copies (both copies represent bisulfite converted promoter sequences) and a 1:1 ratio of methylated LINE1 promoter copies to non-methylated LINE1 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated ELOVL2 promotor and LINE1 promoter of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.

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