Human Cancer Promoter Methylation qPCR Assay Kit

DNA methylation at CpG islands within gene promoter regions plays a key role in the regulation of gene expression. Aberrant promoter methylation, a common epigenetic alteration in cancer, often leads to the silencing of tumor suppressor genes such as RASSF1A, GSTP1, and CDKN2A/p16, which are among the most frequently methylated in human cancers. Hypermethylation of these genes has been implicated in various tumor types and is considered a valuable biomarker for cancer detection, prognosis, and therapeutic monitoring. ScienCell’s Human Cancer Promoter Methylation qPCR Assay Kit (HCPM) is designed to accurately quantify the methylation levels of RASSF1A, GSTP1, and CDKN2A/p16 promoters using bisulfite-converted DNA. The included reference DNA sample contains a 1:1 ratio of methylated to non-methylated promoter sequences for each target gene, serving as a calibration standard for quantifying methylation levels in unknown samples. The assay features highly specific primers that distinguish between methylated and non-methylated DNA, validated through qPCR melt curve analysis, gel electrophoresis, and serial dilution to ensure amplification specificity and efficiency.