大鼠视网膜星形胶质细胞

Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions to their partner neurons in the central nervous system (CNS), such as neuronal guidance during development, nutritional and metabolic support throughout life. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Retinal astrocytes are critical for the homeostasis of neurons and retinal function. Apart from various neuropathological conditions, retinal astrocytes contribute to retinal vascular disease, retinitis pigmentosa and glaucoma. Rat retinal astrocytes (RA-r) are an excellent in vitro model for studying astrocytes under normal physiological and pathological conditions.

RA-r from ScienCell Research Laboratories are isolated from CD® IGS rat retina. RA-r are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RA-r are characterized by immunofluorescence with antibody specific to GFAP. RA-r are negative for mycoplasma, bacteria, yeast, and fungi. RA-r are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing RA-r in vitro.

星形胶质细胞是哺乳动物大脑中的主要细胞类型。它们为中枢神经系统（CNS）中的神经元提供多种支持功能，例如在发育过程中引导神经元、在生命过程中提供营养和代谢支持。大量研究表明，星形胶质细胞是中枢神经系统中功能最为多样化的细胞群之一。在中风或其他损伤过程中，星形胶质细胞功能受损会显著影响神经元的存活。视网膜星形胶质细胞对于维持神经元稳态和视网膜功能至关重要。除了参与多种神经病理条件外，视网膜星形胶质细胞还与视网膜血管疾病、视网膜色素变性以及青光眼相关。大鼠视网膜星形胶质细胞（RA-r）是研究星形胶质细胞在正常生理和病理条件下行为的优秀体外模型。

ScienCell Research Laboratories 提供的 RA-r（大鼠视网膜星形胶质细胞）分离自 CD® IGS 大鼠视网膜。RA-r 在第一代（P1）时进行冷冻保存，并以冻存形式提供。每瓶在 1 ml 体积中含有 >5 x 10⁵ 个细胞。RA-r 通过针对 GFAP 的抗体进行免疫荧光鉴定。经检测，RA-r 不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 提供的培养条件下，RA-r 可保证进一步扩增 5 个细胞世代。

推荐培养基

建议在体外培养 RA-r 时使用 Astrocyte Medium-animal（AM-a，货号：1831）。