成年大鼠星形胶质细胞

Astrocytes are the most abundant cell type in the central nervous system (CNS) and they provide a variety of vital functions for the CNS including: establishment and regulation of the blood brain barrier, functional support for neuronal transmission, survival of neurons, anti-inflammatory responses and wound repair. Astrocytes have also been implicated in various pathological processes such as reactive gliosis. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Rat astrocytes-adult (RA-a) are a useful in vitro model for studying adult glial function and the molecular mechanisms of CNS diseases such as ischemic stroke and multiple sclerosis.

RA-a from ScienCell Research Laboratories are isolated from adult rat brain. RA-a are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RA-a are characterized by immunofluorescence with antibody specific to GFAP. RA-a are negative for mycoplasma, bacteria, yeast, and fungi. RA-a are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing RA-a in vitro.

星形胶质细胞是中枢神经系统（CNS）中最丰富的细胞类型，在维持中枢神经系统正常功能方面发挥着多种关键作用，包括血脑屏障的建立与调控、为神经信号传递提供支持、促进神经元存活、介导抗炎反应以及参与组织损伤修复。

此外，星形胶质细胞还参与多种病理过程，例如反应性胶质增生（reactive gliosis）。在脑卒中及其他神经系统损伤过程中，星形胶质细胞正常功能的受损会显著影响神经元存活。脑损伤后的长期恢复过程，包括神经突起生长、突触可塑性以及神经元再生，也受到星形胶质细胞表面分子表达及营养因子释放的调控。

大量研究表明，星形胶质细胞是中枢神经系统中功能最为多样化的细胞群体之一。成年大鼠星形胶质细胞（RA-a）是研究成年胶质细胞功能以及缺血性脑卒中、多发性硬化等中枢神经系统疾病分子机制的重要 in vitro 模型。

ScienCell Research Laboratories 提供的成年大鼠星形胶质细胞（RA-a）分离自成年大鼠脑组织。细胞于第一代（Passage 1）完成冻存，并以冷冻状态运输。每瓶产品含有超过 5 × 10⁵ 个细胞，包装体积为 1 ml。

RA-a 通过针对 GFAP 的特异性抗体进行免疫荧光鉴定。经检测，产品不含支原体、细菌、酵母及真菌污染。在按照 ScienCell Research Laboratories 提供的培养条件操作时，可保证细胞至少完成 5 次群体倍增。

推荐培养基

建议使用星形胶质细胞培养基-动物源（Astrocyte Medium-animal，AM-a，产品编号 #1831）进行 RA-a 的 体外（in vitro） 培养。