大鼠星形胶质细胞

Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions to their partner neurons in the central nervous system (CNS), such as neuronal guidance during development, nutritional and metabolic support throughout life. Astrocytes have also been implicated in various pathological processes. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Much of what we have learned about astrocytes is from in vitro studies and astrocyte culture is a useful tool for exploring the diverse properties of this cell type.

RA from ScienCell Research Laboratories are isolated from CD® IGS rat brain. RA are cryopreserved as primary cultures and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RA are characterized by immunofluorescence with antibody specific to GFAP. RA are negative for mycoplasma, bacteria, yeast, and fungi. RA are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing RA in vitro.

星形胶质细胞是哺乳动物大脑中的主要细胞类型，在中枢神经系统（CNS）中为神经元提供多种支持功能，包括在发育过程中参与神经元引导，以及在整个生命过程中提供营养和代谢支持。星形胶质细胞还与多种病理过程密切相关。在脑卒中及其他损伤情况下，星形胶质细胞正常功能的受损会显著影响神经元的存活。脑损伤后的长期恢复过程，例如神经突生长、突触可塑性以及神经元再生，也会受到星形胶质细胞表面分子表达及营养因子释放的影响。大量研究表明，星形胶质细胞是中枢神经系统中功能最为多样化的细胞群之一。目前关于星形胶质细胞的大部分认识均来源于体外研究，因此，星形胶质细胞培养已成为探索其多种生物学特性的重要工具。

ScienCell Research Laboratories 的大鼠星形胶质细胞（RA）分离自 CD® IGS 大鼠脑组织。RA 以原代培养形式冻存，并以冷冻状态运输。每瓶含有超过 5 × 10⁵ 个细胞，体积为 1 ml。RA 通过针对 GFAP 的特异性抗体进行免疫荧光鉴定。经检测，RA 不含支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下，RA 可保证继续扩增 5 次群体倍增。

推荐培养基

建议使用 Astrocyte Medium-animal（AM-a，产品编号：1831）进行 RA 的 in vitro 培养。