大鼠小胶质细胞

Microglia, one of the glial cell types in the central nervous system (CNS), is an important integral component of the neuro-glial cell network. They have been observed in the brain parenchyma from the early stage of development to the mature state. Microglia play an important role in brain immune surveillance. They can present antigens in the molecular context of MHC class II expression to CD-4 positive T cells, and are capable of Fc-mediated phagocytosis, and share many common antigens with hemopoietic and tissue macrophages. Upon activation, they act as brain macrophages to clear tissue debris, damaged cells, or microbes, when programmed cell death occurs during brain development or when the CNS is injured. Furthermore, there is evidence that microglia are involved in a variety of physiological and pathological processes in the brain through interaction with neurons, other glial cells, and production of biologically active substances such as growth factors and cytokines.

RM from ScienCell Research Laboratories are isolated from postnatal day 2 CD® IGS rat brain. RM are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. RM are characterized by immunofluorescence with antibody to F4/80. RM are negative for mycoplasma, bacteria, yeast, and fungi. RM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RM are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium
It is recommended to use Microglia Medium (MM, Cat. #1901) for culturing RM in vitro.

小胶质细胞（Microglia）是中枢神经系统（CNS）中的一种胶质细胞，是神经-胶质细胞网络的重要组成部分。从脑发育早期到成熟阶段，均可在脑实质中观察到小胶质细胞的存在。小胶质细胞在脑内免疫监视过程中发挥重要作用。它们能够以主要组织相容性复合体II类（MHC II）分子的形式向CD4阳性T细胞呈递抗原，具有Fc受体介导的吞噬功能，并与造血系统巨噬细胞和组织巨噬细胞共享多种共同抗原。当脑发育过程中发生程序性细胞死亡或中枢神经系统受到损伤时，小胶质细胞被激活并发挥脑内巨噬细胞的作用，清除组织碎片、受损细胞及微生物。此外，越来越多的研究证据表明，小胶质细胞可通过与神经元及其他胶质细胞相互作用，以及分泌生长因子、细胞因子等生物活性物质，参与脑内多种生理和病理过程。

ScienCell Research Laboratories 的大鼠小胶质细胞（Rat Microglia，RM）分离自出生后第2天（P2）的CD® IGS大鼠脑组织。大鼠小胶质细胞（RM）在P0代进行冻存，并以冻存状态提供。每瓶含有超过1 × 10⁶个细胞，悬浮于1 ml溶液中。大鼠小胶质细胞（RM）经F4/80抗体免疫荧光染色鉴定。经检测，RM不含支原体、细菌、酵母和真菌污染。ScienCell Research Laboratories保证RM可在其推荐的培养条件下继续培养；但由于该细胞在常规培养条件下不发生增殖，因此不建议用于扩增培养或长期培养。

推荐培养基
建议使用小胶质细胞培养基（Microglia Medium，MM，产品编号：1901）进行大鼠小胶质细胞（RM）的体外培养。