小胶质细胞是中枢神经系统（CNS）中的一种胶质细胞类型，是神经-胶质细胞网络的重要组成部分。从发育早期到成熟状态，小胶质细胞一直存在于大脑实质中。小胶质细胞在大脑免疫监视中发挥着重要作用。它们可以在MHC II类分子的背景下向CD4阳性T细胞呈递抗原，并能够通过Fc介导的吞噬作用，与造血和组织巨噬细胞共享许多共同抗原。在激活后，它们充当大脑巨噬细胞，清除组织碎片、受损细胞或微生物，尤其是在大脑发育过程中发生程序性细胞死亡或中枢神经系统受伤时。此外，已有证据表明，小胶质细胞通过与神经元、其他胶质细胞的相互作用以及生物活性物质（如生长因子和细胞因子）的产生，参与大脑中的多种生理和病理过程。
ScienCell研究实验室提供的小胶质细胞（MM）来自出生后第2天的CD-1小鼠大脑。小胶质细胞在P0时冷冻保存，并以冷冻状态交付。每个小瓶含有超过1 x 106个细胞，体积为1毫升。小胶质细胞通过使用F4/80和/或CD11b抗体的免疫荧光进行表征。小胶质细胞对支原体、细菌、酵母和真菌均为阴性。小胶质细胞保证在ScienCell研究实验室提供的条件下进一步培养；然而，由于这些细胞在常规培养条件下不增殖，因此不建议用于扩增或长期培养。
推荐培养基
建议使用小胶质细胞培养基（MM，Cat. #1901）在体外培养小胶质细胞。
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M1900
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Endothelial cells lining the vessel network are indispensable for vascular transport but also provide paracrine signals controlling the behavior of nearby cell types. Per... More
Endothelial cells lining the vessel network are indispensable for vascular transport but also provide paracrine signals controlling the behavior of nearby cell types. Pericytes are another essential component of the vessel wall, but little is known about their interactions with other cell populations during organ growth and patterning. Here, we use mouse genetics to address the function of three pericyte-derived factors in postnatal lung and brain. We find that inactivation of the gene for hepatocyte growth factor (HGF) or brain-derived neurotrophic factor (BDNF) in pericytes causes no overt alterations in postnatal brain but impairs lung development, which we attribute to defective interaction with AT2 epithelial cells and pulmonary endothelium, respectively. In contrast, pericyte expression of the growth factor Nodal is dispensable for lung morphogenesis but regulates vessel growth and barrier function in the postnatal brain through interactions with endothelial cells, astrocytes and microglia. Taken together, our findings establish that pericytes are a critical source of paracrine signals controlling morphogenetic processes in an organ-specific fashion. Less
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