大鼠小脑颗粒细胞

The cerebellum is a structure at the base of the brain involved in balance and motor coordination. The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL). The EGL appears segregated during early cerebellum formation and produces only granule cells. Cerebellar granule cells (CGC) are the smallest and most numerous neurons in the brain and play a critical role in motor learning and consolidation. Their axons run as parallel fibres along the coronal axis, and the one-dimensional spread of excitation that results from this arrangement is a key assumption in theories of cerebellar function. During both in vivo and in vitro development, CGC depend on the activity of the NMDA glutamate receptor subtype for survival and full differentiation. Primary rat CGC (RCGC) are used for studying neuronal apoptosis, Batton disease, and fetal alcohol spectrum disorders.

RCGC from ScienCell Research Laboratories are isolated from postnatal day 8 CD® IGS rat cerebellum. RCGC are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. RCGC are characterized by immunofluorescence with antibodies specific to βtubulin III. RCGC are negative for mycoplasma, bacteria, yeast, and fungi. RCGC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RCGC are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.

Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. #1521) for culturing RCGC in vitro.

小脑是位于大脑底部的重要结构，参与平衡控制与运动协调。小脑的发育涉及一系列协调的细胞迁移过程，并包含两个独立的增殖区域：脑室区（ventricular zone）和外颗粒层（external granule cell layer, EGL）。EGL在小脑早期发育阶段逐渐分离形成，并主要产生颗粒细胞。

小脑颗粒细胞（Cerebellar Granule Cells, CGC）是大脑中体积最小且数量最多的神经元类型，在运动学习与记忆巩固过程中发挥关键作用。其轴突沿冠状轴延伸形成平行纤维，这种一维兴奋传播方式是小脑功能理论的重要基础。在体内及体外发育过程中，小脑颗粒细胞依赖NMDA型谷氨酸受体的活性以维持存活并完成正常分化。

原代大鼠小脑颗粒细胞（RCGC）常用于研究神经元凋亡、Batten病以及胎儿酒精谱系障碍等神经系统疾病模型。

ScienCell研究实验室提供的大鼠小脑颗粒细胞（RCGC）来源于出生后第8天（P8）CD® IGS大鼠小脑组织分离获得。RCGC细胞于P0代冻存，并以冷冻状态提供。每支冻存管含>1 × 10⁶个细胞，冻存体积为1 mL。

RCGC通过针对β-微管蛋白III（β-tubulin III）的特异性抗体进行免疫荧光鉴定。经检测，RCGC不含支原体、细菌、酵母及真菌污染。在ScienCell提供的培养条件下可维持存活，但不建议进行扩增或长期培养，因为该细胞在体外不具备增殖能力。

推荐培养基

建议使用神经元培养基（Neuronal Medium，NM，产品编号：1521）进行RCGC体外培养。