CD1小鼠来源脾细胞

The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK T-cells. Primary mouse splenocytes (MS) can be used to isolate CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells.

MS from ScienCell Research Laboratories are isolated from normal adult CD-1 mouse spleen. MS are depleted of splenic macrophages, cryopreserved directly after isolation, and delivered frozen. Each vial contains at least 10 million cells in 1 ml volume. MS are quality control tested for viability. MS are negative for mycoplasma, bacteria, yeast, and fungi. MS can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.

Recommended Medium

It is recommended to use HematoGro Medium (HemGM, Cat. #5501) for short-term maintenance of MS in vitro.

脾脏具有储存和清除红细胞、代谢血红蛋白以及回收铁的重要功能。同时，脾脏在免疫系统中发挥关键作用，可对血液中的抗原产生初级免疫应答并合成抗体。脾细胞（Splenocytes）是来源于脾脏的单核细胞群体，包括T淋巴细胞、B淋巴细胞、NK细胞以及NKT细胞。原代小鼠脾细胞（MS）可用于分离CD4+ T细胞、CD8+ T细胞及CD45R+ B细胞。

ScienCell Research Laboratories 提供的小鼠脾细胞（MS）分离自正常成年 CD-1 小鼠脾脏。该细胞群体已去除脾巨噬细胞，在分离后立即进行冻存，并以冷冻形式提供。每管含有不少于1000万个细胞，体积为1 ml。MS经质量控制检测，具有良好的细胞活力，并确认不含支原体、细菌、酵母及真菌污染。MS可在ScienCell推荐的条件下进行短期体外培养，但不适用于长期培养。

推荐培养基

建议使用 HematoGro 培养基（HemGM，产品编号：5501）用于MS的短期体外维持培养。