C57BL/6小鼠来源脾细胞

The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK Tcells. Primary mouse splenocytes (MS) can be used to isolate CD4+ T-cells, CD8+ T-cells and CD45R+ B cells.

MS from ScienCell Research Laboratories are isolated from normal adult C57BL/6 mouse spleen. MS are depleted of splenic macrophages, cryopreserved after isolation, and delivered frozen. Each vial contains at least 10 million cells in 1 ml volume. MS are quality control tested for viability. MS are negative for mycoplasma, bacteria, yeast, and fungi. MS can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.

Recommended Medium

It is recommended to use HematoGro Medium (HemGM, Cat. #5501) for short-term maintenance of MS in vitro.

脾脏具有储存与清除红细胞、代谢血红蛋白以及回收铁的重要功能。同时，脾脏在免疫系统中发挥关键作用，可对血液中的抗原启动初级免疫应答并产生抗体。

脾细胞（splenocytes）是来源于脾脏的单核细胞群体，主要包括T淋巴细胞、B淋巴细胞、NK细胞以及NK T细胞。原代小鼠脾细胞（MS）可用于进一步分离CD4+ T细胞、CD8+ T细胞以及CD45R+ B细胞等免疫细胞亚群。

ScienCell Research Laboratories 提供的MS来源于正常成年C57BL/6小鼠脾脏，经分离获得。在制备过程中已去除脾脏巨噬细胞，并于分离后进行冻存保存，以冻存状态运输交付。每支冻存管含至少1×10⁷个细胞，体积为1 ml。该产品已通过活力检测质量控制，并确认支原体、细菌、酵母及真菌均为阴性。

MS可在ScienCell Research Laboratories提供的培养条件下进行短期体外维持培养，但不适用于长期传代培养。

推荐培养基

建议使用HematoGro培养基（HemGM，货号#5501）用于MS的体外短期维持培养。