人精囊微血管内皮细胞
分离自人精囊。人精囊微血管内皮细胞(HSVMEC)在第1代培养时进行冻存,并以冷冻状态运输。每支冻存管含有>5 × 105个细胞,体积为1 ml。
The seminal vesicles (SV) are a pair of tubular glands located near the prostate and are essential to the urinary system. They function under androgen control to produce and secrete fluid into the ejaculatory duct. The SV consists of three layers: the inner basal mucosal layer composed of simple cuboidal and pseudo-stratified columnar epithelial cells, the middle muscular layer formed by smooth muscle cells, and the outer layer made up of dense connective tissue. Various pathological conditions can arise in the SV, including congenital SV cysts, seminal vesculitis, and primary and secondary neoplasms. Additionally, SV invasion is often used as a prognostic marker in prostate cancer. SV endothelial cells offer unique opportunities to study many features of the SV.
HSVMEC from ScienCell Research Laboratories are isolated from the human seminal vesicle. HSVMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HSVMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HSVMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HSVMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HSVMEC in vitro.
精囊(SV)是一对位于前列腺附近的管状腺体,对泌尿系统至关重要。在雄激素调控下,精囊产生并将分泌液输送至射精管。SV由三层组成:内层为黏膜基底层,由单层立方上皮细胞和假复层柱状上皮细胞组成;中层为肌肉层,由平滑肌细胞构成;外层由致密结缔组织组成。SV可发生多种病理性疾病,包括先天性SV囊肿、精囊炎以及原发性和继发性肿瘤。此外,精囊侵犯常被用作前列腺癌的预后标志物。SV内皮细胞为研究精囊的多种特征提供了独特的机会。
ScienCell Research Laboratories提供的HSVMEC分离自人精囊。HSVMEC在第1代培养时进行冻存,并以冷冻状态运输。每支冻存管含有 >5 × 105 个细胞,体积为1 ml。HSVMEC采用针对vWF/因子VIII和/或CD31(PECAM1)的特异性抗体进行免疫荧光鉴定。HSVMEC经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。根据ScienCell Research Laboratories提供的培养条件,HSVMEC可保证进一步扩增10个群体倍增。
推荐培养基
建议使用内皮细胞培养基(ECM,产品编号1001)对HSVMEC进行体外培养。
| 目录编号 | 4450 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HSVMEC |
| 规格/数量 | 5 x 10^5 cells/vial. |
| 产品用途 | 本产品仅供研究使用。未经批准,不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后,立即将细胞从干冰转移到液氮中,并在液氮中保存,直到需要进行实验。 |
| 运输 | 干冰。 |
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