The delivery of foreign DNA into eukaryotic cells is one of the most common molecular biology techniques used to study biological mechanisms. However, unlike transformed cell lines, efficient transfection of primary cells can be challenging. EndoFectagen is a cationic polymer-based transfection system specifically designed and optimized for primary endothelial cells. It can be used in the presence of serum and antibiotics and supports multiple culture formats (6-, 12-, 24-, and 96-well plates) with a simple, flexible protocol that is compatible with both serum-containing and serum-free conditions. Instead of a conventional two-day transfection workflow, an optimized one-day procedure can be performed, providing time-saving and highly reproducible transfection.
外源DNA导入真核细胞是研究生物学机制最常用的分子生物学技术之一。然而,与转化细胞系相比,原代细胞的高效转染仍具有较大挑战。EndoFectagen是一种基于阳离子聚合物的转染系统,专为原代内皮细胞设计并优化。该产品可在含血清和抗生素的培养条件下使用,适用于6孔、12孔、24孔和96孔等多种培养板规格,并采用简单、灵活的操作流程,同时兼容含血清和无血清培养条件。相比传统的两天转染流程,EndoFectagen采用优化的一天转染方案,可显著节省实验时间,并实现高效、稳定且具有良好重复性的转染效果。
| 目录编号 | 0903 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | EndoF |
| 规格/数量 | 250 transfections in 96-well plate |
| 产品用途 | 本产品仅供研究使用。未经批准,不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后,将 EndoFectagen A 分装并保存在 -20°C,避免反复冻融。解冻后保存在 4°C 并在一个月内使用。EndoFectagen B 可保存在 4°C。 |
| 运输 | 干冰。 |