人源胎儿心房心脏成纤维细胞

Cardiac fibroblasts (CF) provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. CF proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction and cardiac fibrosis. CF cultures have been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors. CF also have been found to form electric coupling with myocytes through gap junctions exerting electrotonic influences on the cells. These electronic couplings are suggested to play an important role in genesis of complex fractionated atrial electrogram sites and thus are a perfect model for studying atrial fibrillation.

HCF-fa from ScienCell Research Laboratories are isolated from the human fetal atrium. HCF-fa are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HCF-fa are characterized by immunofluorescence with antibody specific to fibronectin. HCF-fa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCF-fa are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing HCF-fa in vitro.

心脏成纤维细胞（CF）为心肌细胞提供结构支持，并在心脏生长以及病理生理状态下负责细胞外基质的合成。CF 是心肌损伤反应中的重要细胞组成部分，也是旁分泌生长因子的来源。CF 的增殖以及基质合成对于心肌梗死部位的瘢痕形成和心脏纤维化至关重要。CF 培养物已被广泛用于研究由生理性（运动）和病理性（高血压）应激因素引起的心脏基质重塑。研究还发现，CF 可通过缝隙连接与心肌细胞形成电耦联，从而对细胞产生电紧张性影响。这些电耦联被认为在复杂碎裂心房电图位点的形成过程中发挥重要作用，因此 HCF-fa 是研究心房颤动的理想模型。

ScienCell Research Laboratories 提供的人源胎儿心房源心脏成纤维细胞（HCF-fa）分离自人源胎儿心房组织。HCF-fa 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HCF-fa 经采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HCF-fa 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HCF-fa 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用成纤维细胞培养基-2（FM-2，产品编号 #2331）对 HCF-fa 进行体外培养。