GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Kit

ScienCell's GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Kit (GQH-TEK) is designed to facilitate gene expression profiling of 88 key genes involved in Tie2 signaling transduction, which is a key component in the formation of blood vessels during angiogenesis. Brief examples of how included genes may be grouped according to function are shown below:

• Ligands & receptors: ANGPT1, ANGPT2, TEK, TIE1
• Morphology: GRB2, PTPN11, SOS1, PTK2, RHOA  
• Motility:  PLD1, MAP2K1, DOK2, NCK1, CRK
• Transcription factors: STAT1, STAT5B, MEF2A, FOXO3, EGR1
• Cell cycle regulation: CDC42, NFKB1, TNIP2, RIPK1, DIABLO

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.